International Journal of Applied Science and Technology

ISSN 2221-0997 (Print), 2221-1004 (Online)

Testing Method for Salinity Tolerance at Germination Stage on Rice Genotypes
Ridwan Diaguna, Faiza Chairani Suwarno, Memen Surahman, Suwarno

Abstract
Salinity is a major constrain to high yield of rice (Oryza sativa L.) grown in coastal area. Testing method for salinity tolerance is performed in a greenhouse by transplanting 14 days-old seedling on soil salinized with 4000 ppm NaCl. The study to develop a testing method for salinity tolerance at germination stage was conducted from September 2016 to March 2017. The germination test with rolled paper towel test treated with 4000 ppm NaCl was identified as the best to differentiate tolerant and susceptible genotypes. The early and green house methods were applied to evaluate the salt tolerance of 40 genotypes. Two weeks old rice seedlings were transplanted into plastic tubs filled with 5 kg of dried soil watered with 4 l of 4000 ppm NaCl solution. The experiment was performed in a RCD with three replications for the green house and a RCBD with three replications for early method. The testing method at germination as well as the vegetative stage could classify the rice genotypes into three groups, tolerant, moderately tolerant, and susceptible. Salinity tolerance at germination stage was closely correlated to those at vegetative stage with 0.71correlation coefficient.

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