International Journal of Applied Science and Technology

ISSN 2221-0997 (Print), 2221-1004 (Online) 10.30845/ijast

Isolation of Twenty-Five New Molecular Microsatellite Markers from Alligator mississippiensis (Alligatoridae, Alligatorinae) EST Sequences using in Silico Approach
Rodrigo Barban Zucoloto, Clara Ribeiro Porto

Microsatellite markers have been applied to conservation genetic studies of crocodilians since the second half of 90's. The identification of highly transferable markers would be very important to crocodilian genetic studies. Here is described the identification of twenty-five new microsatellite markers from Alligator mississippiensis (Daudin, 1802) EST sequences and discussed their expected efficiency for the amplification of DNA of other crocodilian species.

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