International Journal of Applied Science and Technology

ISSN 2221-0997 (Print), 2221-1004 (Online) 10.30845/ijast

EFFECT OF HEAT ON THE ANTIMICROBIAL ACTIVITY OF ALCHORNEA CORDIFOLIA LEAF EXTRACTS.
Gbonjubola O. Adeshina, Akut Stephen, Josiah A. Onaolapo, Joseph A. Ehinmidu, Lilian E. Odama

 

Abstract
Microbial load of the water, methanol, and ethyl acetate extracts of the leaf of Alchornea cordifolia was determined using standard microbiological methods. The water, methanol, ethyl acetate extracts of the leaf of Alchornea cordifolia were subjected to different forms of heat; dry heat, moist heat and sunlight. The leaf was also extracted using hot water- decoction. The antimicrobial activities of the extracts were then investigated against clinical isolates of Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans and the type organisms; Ps. aeruginosa ATCC10145, Staph. aureus ATCC 12600, E.coli ATCC 11775 and C.albicans ATCC 18804 using agar diffusion and broth dilution methods. The microbial load of the fresh leaf extracts ranged between 3.6 x 103 and 6.0 x 104 cfu/ml. The antimicrobial activity of the extracts subjected to moist heat increased more against the organisms especially Staph. aureus and no difference was observed against E.coli when compared with the extracts not subjected to any form of heat. There was a decrease in antimicrobial activity of the extracts exposed to sunlight and no antimicrobial activity was observed in the extracts subjected to dry heat. This study therefore provides a scientific basis for the traditional use of moist heat in the extraction of Alchornea cordifolia leaf.

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